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Background introduction: nctc clone 929 (mouse connective tissue), a clone of cell line 1, was established in March 1948. Cell line 1 is one of the earliest continuously cultured cell lines established, and clone 929 is the earliest clone. From a 100-day-old male c3h/an mouse. Nctc clone 929 cells were the first clone obtained from L cells in March 1948 and one of the first cell lines established in continuous culture. Parental L cells were derived from normal subcutaneous areola and fat of 100-day-old male c3h/an mice. Cells are passaged according to a certain seeding ratio, and the first passage is 1:3. If the cells are full within two days, the passage ratio can be increased. If the cells are not full after three or four days of growth, the passage ratio can be appropriately reduced.

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